Jeong DW, Park W, Bedair TM, Kang EY, Kim IH, Park DS, Sim DS, Hong YJ, Koh WG, Jeong MH, Han DK
Augmented re-endothelialization and anti-inflammation of coronary drug-eluting stent by abluminal coating with magnesium hydroxide.
Biomaterials Science
Drug-eluting stent (DES) has been widely used as a treatment approach for coronary artery diseases. Generally, conventional DES was fully covered with drugs and biodegradable polymers on both abluminal and luminal layers (i.e., conformal coating). However, uncontrolled drug release from the luminal drug-coating layer of the stent is known to inhibit re-endothelialization. Furthermore, the acidification of surrounding tissue by decomposed coating polymer causes inflammation, resulting in restenosis and late thrombosis. To overcome these limitations, here we demonstrated a functional DES coated with poly(lactic-co-glycolic acid) (PLGA), sirolimus (SRL), and magnesium hydroxide (Mg(OH)2, MH) precisely only on the abluminal layer. The acidic neutralization effect of MH was elucidated by measuring pH change of the fabricated film in PBS solution. In in vitro cell study, the stent coated with MH exhibited higher compatibility with human coronary artery endothelial cells (ECs) and lower inflammation score as compared to control stent. Finally, in vivo large porcine model, the abluminal coated DES with SRL and MH showed excellent re-endothelialization as well as anti-inflammatory and anti-thrombotic effects. In conclusion, it is believed that this approach has great potential for the development of functional DES for the treatment of cardiovascular diseases.
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DATE : 21-06-10 11:46
HIT : 1,361
