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논문번호 74
논문제목(영문) Therapeutic effect of Akt1 siRNA Nanoparticle Eluting Coronary Stent on Suppression of Post-angioplasty Restenosis.
국내외구분 국외 SCI여부 SCI(E)
연구책임자역할 공저자
주저자명 Che HL
교신저자명 Park IK, Ahn Y
공동저자명 Che HL, Bae IH, Lim KS, Song IT, Lee H, Lee D, Kim WJ, Jeong MH, Park IK, Ahn Y;
게제년월일 2016-06-01
ISSN 1550-7033
Impact Factor 5.338
학술지명 Journal of Biomedical Nanotechnology
서지사항 0집 / 12권 / 6호,   페이지(1 - 12)
요약초록문
(Abstract) 입력		 For effective treatment of restenosis, therapeutic genes are delivered locally from a coated stent at the site of injury, leading to inhibition of smooth muscle proliferation and neo-intimal hyperplasia while promoting re-endothelialization. In a previous study, we delivered Akt1 siRNA nanoparticles (ASNs) from a hyaluronic acid (HA)-coated stent surface to specifically suppress the pro-proliferative Akt1 protein in smooth muscle cells (SMCs). In the present study, therapeutic efficacy was investigated in a rabbit restenosis model after percutaneous implantation of an ASN-immobilized stent in a rabbit iliac artery. Quantitative and qualitative analyses of in-stent restenosis were investigated in an in vivo animal model by micro-CT imaging and SEM observation, respectively. Proliferation status and neo-intima formation of the vascular tissues located near ASN-immobilized stents were analyzed by immunohistochemical staining using anti-Akt1 and anti-Ki67 antibodies and histological analyses, such as hematoxylin and eosin staining and Verhoeff’'s elastic stain. Re-endothelialization after implantation of an ASN-immobilized stent was also analyzed via immunohistochemistry using an anti-CD31 antibody. To elucidate the molecular mechanism related to reducing SMC proliferation and subsequent inhibition of in-stent restenosis in vivo, protein and mRNA expression of Akt1 and downstream signaling proteins were analyzed after isolating SMC-rich samples from the treated vasculature. The implanted Akt1 siRNA-eluting stent efficiently mitigated in-stent restenosis without any side effects and can be considered a successful substitute to current drug-eluting stents.
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